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Low Volume Flow Cell

MicroLWCC - Low Volume Flow Cell

The WPI MicroLWCC small volume flow cell increases the optical path length for measurements by 10, 50 or 100 mm. Suitable for FIA, GFIA, HPLC and optofluidics.

For FIA, HPLC and process analysis.

Key features

  • Flow cell for absorbance in UV/VIS
  • Minimal interior volume
  • Fits 500 and 600 µm fibers [Web part # 505195 Catalog part # WVLUXUVIS-S-0600-100-SMA]
  • High UV transmission
  • 0–10 mL/min flow rate

Benefits

  • A high-performance coupler
  • Offset for low refractive indices
  • Complies with TIDAS systems and other fiber-coupled spectroscopic machinery

Applications

  • FIA, GFIA, HPLC, Optofluidics
  • Process control
  • DNA, RNA and protein quantification
  • Analysis of nutrients and trace metals using colorimetry
  • Drug development and testing for dissolution

A novel fiber optic low volume flow cell for UV/VIS/NIR absorbance analysis is called the MicroLWCC. The analyte solution serves as the core of a fluid-filled light waveguide, which is based on the well-known liquid core waveguide technology from WPI. PEEK, fused silica, and PTFE are the wetted components in the sample cell light path. Light is transported to and from the sample cell using optical fibers. The cell can be utilized in biochemistry for HPLC analysis, drug discovery and dissolution testing, colorimetric nutritional and trace metal analysis, DNA, RNA, and protein quantification, and process control.

  LWCC-M-10 LWCC-M-50  LWCC-M-100
Optical Pathlength 10 mm  50 mm  100 mm
Internal Volume 2.4 μL 12 µL  24µL 
Refractive Index@ 280nm (measured using ASTM E 685-93) < 7 mAU <15 mAU  <30 mAU 
Transmission @ 254nm (Reference:2 600μm fiber, butt-connected) 25% 20%  15% 
Transmission @ 500nm (Reference:2 600μm fiber, butt-connected)  40% 35%  30% 
Wavelength Range 200-1000 nm  
Fiber Connection [μm] 600 (SMA)  
Maximum Pressure 1000 PSI
Wetted Materials PEEK, Fused Silica, PTFE  

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