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npi TEC-10CX

TEC-10CX – Two Electrode Clamp for Oocytes

NPI's TEC-10CX serves as a two-electrode voltage clamp amplifier designed for advanced recordings from oocytes.

Advantages of the TEC-10CX

  • The fastest oocyte clamp system currently available in the market.
  • Simple setup – no requirement for a 'virtual ground' electrode.
  • Enhanced performance attributed to NPI's PI control technology.
  • A more basic version is also offered: TEC-03X

Features:

  • An advanced and exceptionally rapid two-electrode voltage and current clamp (V/C) amplifier featuring a PI-controller for oocyte recordings.
  • Convenient push buttons for quick and easy selection of the OPERATION MODE.
  • Differential potential registration along with a high-voltage current source output, including an automated electrode resistance testing mode that can be utilized even when electrodes are inserted in an oocyte.
  • VC OUTPUT LIMITER, COMMAND FILTER, INTEGRATOR, and SERIES RESISTANCE COMPENSATION for precise adjustments of the VC circuit.
  • Current transient compensation safeguards the data acquisition system from clipping.
  • Digital DISPLAYS for monitoring current, voltage, and electrode resistance.
  • Four-pole BESSEL filter for current processing.
  • No virtual ground is necessary for capturing membrane currents.
  • The OSCILLATION SHUT-OFF unit protects cells from potential damage.
  • The standard current range is ±150 µA into 1 MΩ. Current headstages with selectable current ranges (x0.1, x1, x2, x5 or x0.1, x0.2, x0.5, x1) are also available.
  • User-friendly operation with all leading data acquisition systems, remote selection of OPERATION MODE, and telegraphing (monitoring) outputs for current sensitivity and filtering. 

 

Feature

TEC-03X

TEC-05X

TEC-10CX

VC-Error display

X

X

PI-Controller

X

X

X

Series Resistance Compensation

X

X

X

Electrode Resistance Test

X

X

X

Mode of Operation Switch

Rotary switch

Rotary switch

Push buttons

Bridge Mode

X

Lowpass filter: 20 … 20 kHz

Four-Pole BESSEL

Four-Pole BESSEL

Four-Pole BESSEL

Direct Current output from Headstage

X

X

X

Oscillation Shut-off

X

X

X

Transient Compensation

X

Capacity Compensation

X

X

X

Cap. Comp. of Current Headstage

X

Please be advised: These are the standard configurations available. Should you have specific requirements, we encourage you to reach out to us directly.

Specifications of the TEC-10CX

MODES OF OPERATION:

  • RPEL: Electrode resistance test potential electrode
  • VC: Voltage Clamp mode
  • CC: Current Clamp mode
  • RCEL: Electrode resistance test current electrode

HEADSTAGES 

Potential headstage:

  • Differential input (for suppression of bath potentials), cmr > 80 dB; Input resistance: >1013 Ω; operating voltage ±15 V
  • Electrode connector: BNC with driven shield; driven shield range: ±15 V, output impedance 250 Ω
  • Reference connector (bath): gold-plated SMB, grounded shield
  • Ground connector: 2.3 mm connector or headstage enclosure
  • Size: 65x25x25 mm, headstage enclosure is connected to ground
  • Holding bar: diameter 8 mm, length 10 cm

Current headstage:

  • Operating voltage range: ±150 V
  • Input resistance: >1012 Ω (can be internally trimmed)
  • Electrode connector: gold-plated SMC connector, grounded shield
  • Power dissipation: 6 W
  • Size: 100x55x35 mm, grounded enclosure

Current range: 

  • 150 µA into 1 MΩ (standard)
  • Current range switch (optional): x0.1, x1, x2, x5 (other ranges available)

Bandwidth and speed of response:

  • Full power bandwidth (REL = 0): >100 kHz
  • Rise time (10-90%): <30 µs (current pulse of 100 µA applied to REL = 1 MΩ)
  • Bandwidth switch: wide band or 10 Hz for simultaneous patch clamp recordings

Current electrode parameter controls:

  • Leakage current: adjustable to zero with ten-turn control
  • Offset compensation: ±500 mV, ten-turn control

Potential electrode parameter controls:

  • Capacity compensation: range 0-30 pF, ten turn control
  • Offset compensation: ±300 mV, ten-turn control

POTENTIAL OUTPUTS:

  • Potential electrode: two outputs, sensitivity x10 mV and x40 mV, output impedance 50 Ω; output voltage range ±15 V
  • Current electrode: sensitivity x10 mV; output impedance 250 Ω,
  • output voltage range ±15 V
  • DISPLAY (switch selected): XXX mV

AUDIO MONITOR: Pitch correlated with potential signals

OSCILLATION SHUT-OFF: Turns off current injection and capacity compensation, function indicated by red / green LED, disabled / off / reset switch, threshold set with linear control (0-1200 mV)

ELECTRODE RESISTANCE TEST (both electrodes): 100 mV / MΩ, obtained by application of square current pulses ±10 nA, display XX.X MΩ, selected automatically

CURRENT OUTPUTS:

  • Uncompensated output signal: sensitivity 0.1 V/µA, output impedance 50 Ω, output voltage range ±15 V
  • Compensated /filtered output: sensitivity: 0.1…10 V / µA (0.1, 0.2, 0.5, 1, 2, 5, 10 V / µA) steps selected by rotary switch, with low-pass Bessel filter, output impedance 50 Ω
  • Sensitivity monitor: +1…+7 Volt, 1V / switch position, output impedance 50 Ω
  • DISPLAY: X.XX µA

CURRENT OUTPUT FILTERS:

  • Four-pole low-pass Bessel filter
  • 16 corner frequencies: 20, 50, 100, 200, 300 500, 700, 1k, 1.3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k Hz.
  • Frequency monitor: -8…+7 V, 1 Volt / switch position, output impedance 50 Ω

CURRENT CLAMP:

  • Inputs: 1 µA /V
  • Input resistance: >100 kΩ
  • HOLD: X.XX µA, ten-turn digital control with -/0/+ switch, maximum 10 µA.

Noise (in current clamp):

  • Potential output: 100 µV pp
  • Current output: 200 pA pp with 1 MΩ resistance and 10 kHz bandwidth (internal four-pole Bessel filters)

Speed of response (in current clamp):

  • (1% settling time; potential output signals after application of square pulses of 1V with 1 MΩ electrode resistance)
  • Potential electrode: <10 µs
  • Current electrode: <50 µs

VOLTAGE CLAMP:

  • Input sensitivity: :10 mV
  • Input resistance >100 kΩ
  • HOLD: XXX mV, ten-turn digital control with +/0/- switch, maximum 1000 mV
  • RISE TIME LIMIT: 0-0.2 ms
  • GAIN: 10 µA/V – 10000 µA/V , ten-turn linear control.
  • INTEGRATOR TIME CONSTANT: 100 µs – 10 ms, potentiometer
  • OUTPUT CURRENT LIMIT: 0-100% linear control.

Noise (in voltage clamp):

  • filters set to 10 kHz, other settings see below
  • Potential output: <100 µV pp
  • Current output: <10 nA pp at 10 kHz, < 2 nA at 500 Hz

Speed of response (in voltage clamp): 1 % settling time: < 80 µs for 10 mV step and < 100 µs for 100 mV step applied to cell model (Re = 1 MΩ, Rm = 100 kΩ, Cm = 0.1 µF)

POWER REQUIREMENTS: 115/230 V AC, 60 W (1.25 / 0.63 A fuse)

DIMENSIONS: 19″ rackmount cabinet, 19″ (483 mm) wide, 14″ (355 mm) deep, 5.25″(132.5 mm) high

WEIGHT: 7 kg

Ordering Information

  • TEC-10CX TEC-10CX Oocyte Clamp with Digital Filter, Gain & Transient Compensation
  • TEC-10X TEC-10CX Oocyte Clamp without Transient Compensation, with Digital Filter, Gain
  • TEC-EH PPH-0P-BNC Microelectrode Holder 1.5 mm glass, w/o. port, w. BNC connector
  • TEC-EH-SET Electrode Holder Set for TEC Amplifiers
  • TEC-MOD Passive Cell Model for TEC Systems
  • TEC-CON SubClic Cable for TEC Headstage
  • TEC-CHS-4 Current Headstage with four Ranges x0.1,x1,x2,x5
  • TEC-CHS-4S Current Headstage with four Ranges x0.1,x0.2, x0.5, x1
  • TEC-RS-R Additional system for measuring series resistance in Oocytes

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