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npi TEC-03X

TEC-03X – Two Electrode Clamp for Oocytes

npi's TEC-03X is two-electrode voltage clamp amplifier for standard recordings from oocytes (a straightforward version of the TEC-10CX).

Advantages of the TEC-03X

  • Reduce expenses with this more straightforward version of the TEC-10CX
  • User-friendly oocyte recording amplifier
  • Rapid clamp speed and enduring stability

Features:

  • The TEC-03X employs the conventional two-electrode method and serves as an excellent, user-friendly system for oocyte recordings
  • Precise and swift two-electrode voltage and current clamp (V/C) amplifier featuring a PI-controller for examining substantial membrane currents
  • Differential potential measurement and high-voltage current source output, with an automated electrode resistance testing mode that can be utilized even when electrodes are inserted in an oocyte
  • Digital DISPLAYS for current, voltage, and electrode resistance
  • Four-pole BESSEL filter for current
  • No virtual ground is required for recording membrane currents
  • OSCILLATION SHUT-OFF unit safeguards cells from damage
  • The standard current range is ±150 µA into 1 MΩ. Current headstages with selectable current ranges (x0.1, x1, x2, x5 or x0.1, x0.2, x0.5, x1) are also available
  • Simple operation with all primary data acquisition systems, remote selection of MODE of OPERATION (CC, VC), and telegraphing (monitoring) outputs for current sensitivity and filtering.

Feature

TEC-03X

TEC-05X

TEC-10CX

VC-Error display

X

X

PI-Controller

X

X

X

Series Resistance Compensation

X

X

X

Electrode Resistance Test

X

X

X

Mode of Operation Switch

Rotary switch

Rotary switch

Push buttons

Bridge Mode

X

Lowpass filter: 20 … 20 kHz

Four-Pole BESSEL

Four-Pole BESSEL

Four-Pole BESSEL

Direct Current output from Headstage

X

X

X

Oscillation Shut-off

X

X

X

Transient Compensation

X

Capacity Compensation

X

X

X

Cap. Comp. of Current Headstage

X

Please note: These are the available standard configurations. If you have special requirements, please contact us directly.

Specifications of the TEC-03X

MODES OF OPERATION

  • VC: Voltage Clamp mode
  • OFF: Membrane potential recording only
  • CC: Current Clamp mode
  • EXT (TTL): External control by TTL
  • MODE selection: toggle switch, LED indicators; remote selection by TTL pulse

HEADSTAGES 

Potential headstage:

  • Differential input (for suppression of bath potentials), cmr > 80 dB; Input resistance: >1013 Ω; operating voltage ±15 V
  • Electrode connector: BNC with driven shield; driven shield range: ±15 V, output impedance 250 Ω
  • Reference connector (bath): gold-plated SMB, grounded shield
  • Ground connector:  2.3 mm connector or headstage enclosure
  • Size:  65x25x25 mm, headstage enclosure is connected to ground
  • Holding bar:  diameter 8 mm, length 10 cm

Current headstage:

  • Operating voltage range: ±150 V
  • Input resistance: >1012 Ω (can be internally trimmed)
  • Electrode connector: gold-plated SMC connector, grounded shield
  • Power dissipation: 6 W
  • Size:   100x60x40 mm, grounded enclosure

Current range:

  • 150 µA into 1 MΩ (standard)
  • Current range switch (optional): x0.1, x1, x2, x5 (other ranges available)

Bandwidth and speed of response:

  • Full power bandwidth (REL = 0): >100 kHz
  • Rise time (10-90%):  <30 µs (current pulse of 100 µA applied to REL = 1 MΩ)
  • Bandwidth switch:  wide band or 10 Hz for simultaneous patch clamp recordings

Current electrode parameter controls:

  • Leakage current:  adjustable to zero with ten-turn control
  • Offset compensation:  , ±500 mV, ten-turn control

Potential electrode parameter controls:

  • Capacity compensation:  range 0-30 pF, ten turn control
  • Offset compensation:  ±300 mV, ten-turn control

POTENTIAL OUTPUTS:

  • Potential electrode: sensitivity x10 mV, output impedance 50 Ω; output voltage range ±15 V
  • Current electrode:  sensitivity x10 mV; output impedance 250 Ω, output voltage range ±15 V
  • DISPLAY (switch selected): XXX mV

AUDIO MONITOR: Pitch correlated with potential signals

OSCILLATION SHUT-OFF: Turns off current injection and capacity compensation, function indicated by red / green LED, disabled / off / reset switch, threshold set with linear control (0-1200 mV)

ELECTRODE RESISTANCE TEST (both electrodes): 100 mV / MΩ, obtained by application of square current pulses ±10 nA, DISPLAY (switch selected): XX.X MΩ

CURRENT OUTPUTS:

  • Uncompensated output signal: sensitivity 0.1 V/µA, output impedance 50 Ω, output voltage range ±15 V
  • Compensated /filtered output: sensitivity: 0.1…10 V / µA (0.1, 0.2, 0.5, 1, 2, 5, 10 V / µA) steps selected by rotary switch, with low-pass Bessel filter, output impedance 50 Ω
  • Sensitivity monitor:  +1…+7 Volt, 1V / switch position, output impedance 50 Ω
  • DISPLAY:   X.XX µA

CURRENT OUTPUT FILTERS:

  • Four-pole low-pass Bessel filter
  • 16 corner frequencies: 20, 50, 100, 200, 300 500, 700, 1k, 1.3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k Hz.
  • Frequency monitor: -8…+7 V, 1 Volt / switch position, output impedance 50 Ω

CURRENT CLAMP: 

  • Inputs:  1 µA /V
  • Input resistance: >100 kΩ
  • HOLD:  X.XX µA, ten-turn digital control with -/0/+ switch, maximum 10 µA.

Noise (in current clamp):

  • Potential output: 100 µV pp
  • Current output: 200 pA pp with 1 MΩ resistance and 10 kHz bandwidth (internal four-pole Bessel filters)

VOLTAGE CLAMP:

  • Input sensitivity:   :10 mV
  • Input resistance   >100 kΩ
  • HOLD:    XXX mV, ten-turn digital control with +/0/- switch, maximum 1000 mV
  • GAIN:    10 µA/V – 10000 µA/V , ten-turn linear control.
  • INTEGRATOR TIME CONSTANT: 100 µs – 10 ms, potentiometer.

Noise (in voltage clamp):

  • filters set to 10 kHz, other settings see below
  • Potential output:   <100 µV pp
  • Current output:   <10 nA pp at 10 kHz, < 2 nA at 500 Hz

POWER REQUIREMENTS: 115/230 V AC, 60 W (1.25 / 0.63 A fuse, slow)

DIMENSIONS: 19″ rackmount cabinet, 19″ (483 mm) wide, 14″ (355 mm) deep, 5.25″(132.5 mm) high

WEIGHT: 8 kg

Ordering Information

  • TEC-03 TEC-03X Oocyte Voltage/Current Clamp Amplifier
  • TEC-EH PPH-0P-BNC Microelectrode Holder 1.5 mm glass, w/o. port, w. BNC connector
  • TEC-EH-SET Electrode Holder Set for TEC Amplifiers
  • TEC-MOD Passive Cell Model for TEC Systems
  • TEC-CON SMB Cable for TEC Potential Headstage (REF)

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