TEC-05X – Two Electrode Clamp for Large Cells

NPI's TEC-05X serves as a two-electrode voltage clamp amplifier designed for recordings from small to medium-sized cells.

Advantages of the TEC-05X

Optimal amplifier for medium-sized cells, including invertebrate ganglion cells, muscle cells, or neuromuscular junction preparations.
Compatible with sharp microelectrodes and patch pipettes in both whole-cell and perforated-patch configurations.
Single electrode recording in BRIDGE mode: Authentic current clamp operation with recorded membrane potential and complete cancellation of series resistance (potential electrode).
Four-pole BESSEL filter for current.
Digital DISPLAYS for current, voltage, and electrode resistance.
BUZZ and ELECTRODE CLEAR feature with remote hand or foot switch.
OSCILLATION SHUT-OFF unit safeguards cells from damage.
Available in low voltage (±15 V) and high voltage (up to ±150 V) versions, ranging from pA to µA.
Simple connection to all major data acquisition systems, TTL control of MODE of OPERATION, and telegraphing outputs for current sensitivity and filter.

Feature	TEC-03X	TEC-05X	TEC-10CX
VC-Error display	–	X	X
PI-Controller	X	X	X
Series Resistance Compensation	X	X	X
Electrode Resistance Test	X	X	X
Mode of Operation Switch	Rotary switch	Rotary switch	Push buttons
Bridge Mode	–	X	–
Lowpass filter: 20 … 20 kHz	Four-Pole BESSEL	Four-Pole BESSEL	Four-Pole BESSEL
Direct Current output from Headstage	X	X	X
Oscillation Shut-off	X	X	X
Transient Compensation	–	–	X
Capacity Compensation	X	X	X
Cap. Comp. of Current Headstage	–	X	–

Please be advised: These are the standard configurations available. Should you have specific requirements, do not hesitate to contact us directly.

Potential headstage:
Operating voltage ±15 V;
Size (approx.): 70x26x26mm, holding bar diameter 8 mm, length 150 mm;
Input resistance: >1013 Ω;
Differential input (for supression of bath potentials): cmr >80 dB.Current headstage (15 V, TEC-05X)):
Operating voltage: ±15 V;
Size (approx.): same as potential headstage,
Input resistance: >1012 Ω (internally trimmable).Current headstage (150 V):
Operating voltage: ±150 V (standard);
Size (approx.): 100x50x30 mm, grounded enclosure,
holding bar diameter 8 mm, length 10 cm (isolated from ground, standard system only);
Input resistance: >1012 Ω (internally trimmable);
Power dissipation: 6 W (standard)

CURRENT OUTPUTS:

Uncompensated output signal: sensitivity 0.1 V/µA or 0.1 nA/V (TEC-05X), voltage range ±15 V;
Compensated/filtered output: sensitivity: 0.1 V…10 V/µA (V/nA for TEC-05X)

POTENTIAL OUTPUTS:

Potential electrode: two outputs, sensitivity x10 mV only (TEC-03X) or x10 mV and x40 mV (TEC-05X, TEC-10CX), voltage range ±15V;
Current electrode: sensitivity x10 mV, voltage range ±15 V;

CURRENT CLAMP (standard headstage):
Inputs: 1 µA/V, 0.1 µA/V with ON/OFF switches (TEC-10CX), 1 µA/V (TEC-03X), 1 nA/V, 0.1 nA/V with ON/OFF switches (TEC-05X);
input resistance >100 kΩ;
VOLTAGE CLAMP:
Inputs: :10 mV and :40 mV (TEC-05X, TEC-10CX) with ON/OFF switches, input resistance >100 kΩ;

MODE selection: rotary switches (TEC-03X, TEC-05X), or pushbuttons (TEC-10CX) , LED indicators;
remote selection by TTL inputs, software assessable (optional).
Current Electrode Parameter Controls:
Offset compensation: ten-turn control, ±1200 mV;
Capacity compensation (optional, TEC-05X): range 0 – 30 pF, ten-turn potentiometer.
Potential Electrode Parameter Controls:
Offset compensation: ±200mV, ten-turn control;
Capacity compensation: range 0 – 30 pF, ten-turn control.
Oscillation Shut off: disabled / off / reset switch, threshold set with linear control (0- 1200 mV).
Current Clamp: HOLD: X.XX µA (TEC-03X, TEC-10CX), X.XX nA, ten-turn digital control with -/0/+ switch, maximum 10 µA (TEC-03X, TEC-10CX) or 10 nA (TEC-05X);
BRIDGE balance (optional, TEC-05X systems): max. 10 MΩ or max. 100 MΩ (switch selected) with ten-turn digital control.
INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ;
Voltage Clamp: HOLD: XXX mV, ten-turn digital control with +/0/- switch, maximum 1000 mV;
RISE TIME LIMIT: 0 – 0.2 ms;
GAIN: 10 µA/V – 10000 µA/V, ten-turn linear control;
INTEGRATOR TIME CONSTANT: 200 µs – 2 ms, ten-turn control (TEC-03X) or control with ON/OFF switch (TEC-05X, TEC-10CX) ;
OUTPUT CURRENT LIMIT5 (TEC-10CX): 0 – 100%, linear control;

OUTPUT FILTERS: Current output: lowpass filters with 16 corner frequencies (20 Hz – 20 kHz)

AUDIO MONITOR: Pitch correlated with potential signal.

POWER REQUIREMENTS: 115 V/230 V AC, 60 W (1.25 A/0.63 A fuse, SLOW).

DIMENSIONS: 19″ rackmount cabinet, 19″ (483 mm) wide, 14″ (355 mm) deep, 5.25″(132.5 mm) high

Weight: approx. 8 kg.

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