Advantages of the SEC-03M:
- Eliminate errors caused by series resistance
- Perfect for perforated patch clamp or
- Recording with sharp microelectrodes
- Also offered as a 19″ stand-alone device
Single Electrode Clamp
The SEC-03M is a switched mode single electrode voltage and current clamp amplifier module designed for recordings utilizing sharp microelectrodes or suction (patch) pipettes.
It is now feasible to integrate up to three SEC amplifiers within a single 19 inch housing for the study of coupled cells, and when utilizing npi’s software CellWorks, no BNC cabling is necessary. All signals are internally connected to the INT-20M breakout box through the EPMS bus.
Alternatively, one can combine an SEC-03M with a PEN-03M module for straightforward cell penetration or with a HVC-03M module to achieve both a single and a two-electrode voltage-clamp system within one EPMS housing.
Most suitable for measuring coupled cells
The integration with PEN-03M and HVC-03M modules creates a flexible system capable of
- Recording with sharp microelectrodes
- Recording with patch pipettes in whole-cell or perforated patch configuration
- Single electrode recording (CC and VC) independently from two cells
- Two electrode recording (CC and VC) from one cell
- Ultra-fast Iontophoresis
Specifications of the SEC-03M:
MODES OF OPERATION:
- VC: Voltage Clamp mode (discontinuous)
- CC: Current Clamp mode (discontinuous)
- OFF: Current- and Voltage Clamp disabled
- BR: Bridge Mode (continuous CC)
- EXT: External control mode
- MODE selection: toggle switch, LED indicators
HEADSTAGES:
- Standard headstage
- Operation voltage: ±15 V
- Input resistance: <1013 Ω (internally adjustable)
- Current range (continuous mode): 120 nA into 100 MΩ
- CC control: Coarse control for input capacity compensation
- Electrode connector: gold plated SUBCLIC (SMB) with driven shield
- Driven shield output: 2.3 mm connector, range ±15 V, impedance 250 Ω
- Ground: 2.3 mm connector or headstage enclosure
- Holding bar: diameter 8 mm, length 150 mm
- Size: 100x40x25 mm
- Headstage enclosure is connected to groundLow noise (whole-cell) headstage (SEC-HSP)
- Operation voltage: ±15 V
- Input resistance: <1013 Ω (internally adjustable)
- Current range (continuous mode): 12 nA into 100 MΩ
- external CC control: Coarse control for input capacity compensation
- Electrode connector: BNC connector with driven shield
- Driven shield output: 1 mm connector, range ±15 V, impedance 250 Ω
- Ground: 1 mm connector or headstage enclosure
- Mounting plate: 60×50 mm with four 6 mm holes
- Headstage enclosure is connected to groundDifferential input headstage (SEC-HSD)
- Operation voltage: ±15 V
- Input resistance: <1013 Ω (internally adjustable)
- CMR: >90 dB
- Current range (continuous mode): 120 nA into 100 MΩ
- CC control: Coarse control for input capacity compensation
- Electrode connectors: two gold plated SUBCLIC (SMB) with driven shields
- Driven shield output: 2.3 mm connector, range ±15 V, impedance 250 Ω
- Ground: 2.3 mm connector or headstage enclosure
- Holding bar: diameter 8 mm, length 100 mm
- Size: 100x40x25 mm
- Headstage enclosure is connected to ground
ELECTRODE PARAMETER CONTROLS:
- Offset: ten-turn control, ±200 mV
- Capacity compensation: range 0-30 pF
- adapts compensation circuit to electrode parameters
- coarse control at headstage
- fine control at front panel: ten-turn potentiometer
BANDWIDTH and SPEED OF RESPONSE:
- Full power bandwidth (REL = 0): >100 kHz
- Rise time (10-90%, REL = 100 MΩ): <30 µs
- Rise time (10-90%, REL = 5 MΩ): <8 µs
- Electrode artifact decay
- (switched modes, 10 nA signal): <1 µs (REL = 5 MΩ); <1.5 µs (REL = 100 MΩ) CAPACITY COMPENSATION tuned with no overshoot.
ELECTRODE RESISTANCE TEST: obtained by application of square current pulses ±1 nA; 10 mV/MΩ; display XXX MΩ
CELL PENETRATION: Overcompensation of capacity compensation, fixed timed
SWITCHED MODES PARAMETERS: Switching frequency: linear control, 2-40 kHz; duty cycle: fixed to ¼ (25% current injection)
CURRENT RANGE in SWITCHED MODE:
- Standard headstage: ±30 nA
- SEC-HSP headstage: ±3 nA
SWITCHED MODE OUTPUTS:
- Electrode potential: max. ±12 V, output impedance: 250 Ω
- Switching frequency: TTL, output impedance: 250 Ω
CURRENT OUTPUT: 10 nA / V; output impedance: 250 Ω; current display: X.XX nA
POTENTIAL OUPUT: Sensitivity: x10 mV; output impedance: 250 Ω; potential display: XXX mV
CURRENT CLAMP:
- Input: 1 nA/V; input resistance: >100 kΩ
- HOLD: X.XX nA, ten-turn digital control with -/0/+ switch, max. 10 nA
- BRIDGE balance : XXX MΩ with ten-turn digital control
- Noise (BRIDGE MODE): 400 µVpp / pApp with 100 MΩ resistance at 10 kHz bw
VOLTAGE CLAMP:
- Input: /10 mV; input resistance >100 kΩ
- HOLD: XXX mV, ten-turn digital control with +/0/- switch, max. 1000 mV
- GAIN: 100 nA/V – 10 µA/V ten-turn linear control
- Noise: potential output: <400 µVpp, current output: <400 pApp.
SPEED of RESPONSE (VC Mode):
- 1 % settling time: <80 µs for 10 mV step and <800 µs for 50 mV step applied to a cell model
- (REL = 100 MΩ, Rm = 50 MΩ, Cm = 470 pF, switching frequency = 30 kHz, standard headstage)
DIMENSIONS: 24 HP (121.5 mm) x 3U (128.5 mm) x 7 inch (175 mm) deep
Ordering Information
- SEC-03M SEC-03M System for EPMS
- SEC-HS SEC-HS Standard Headstage
- SEC-HSD SEC-HSD Headstage with differential input
- SEC-HSP SEC-HSP Low-noise Headstage
- SEC-MINI-SE SEC-MINI Headstage standard cable 2,5m, D=2.3mm, 12x10mm w/ Housing
- SEC-MINI-C Standard Cable Extension (max. 6m)
- SEC-MINI-SC Special Cable for MINI-Headstage, high flexibility, 6-11 lines, 2.5m
- SEC-MINI-SCE Special Cable Extension for MINI-Headstage, high flexibility, 6-11 lines, max. 6m
- SEC-EH Standard Micro-/Patch Electrode Holder
- SEC-EH-SET Electrode Holder Set for SEC Amplifiers
- SEC-MODA Cell Model w/ Active Memb.Res.
- SEC-MOD SEC Cell Model Circuit
- SEC-MOD-D Dual SEC Cell Model Circuit
- SEC-CON SMB Cable for SEC Headstage
