C100-Pro Automated Cell Counter

Your Research will be Accelerated by a True Time-Saver

With its superior microscope optics structure and extremely sophisticated analytical software, the RWD automated cell counter is a dependable tool for counting cells.

The C100-Pro software and data management solution completely complies with FDA 21 CFR Part 11 to fulfill the demands of biopharma and cell therapy.

Multiple application scenarios are realized by the RWD C100-Pro Automatic Cell Counter.

• Cell Count Analysis: Suitable for counting most cell lines, stem cells, primary cells, and other particles with a diameter of 4–60 μm.
• Cell Viability Analysis: After staining, live cells are circled in green and dead cells are circled in red while analyzing cell viability in trypan blue mode.
• Analysis of Cell Size: One important aspect of cell research is cell size, which is frequently assessed in drug testing, cell viability, and transfection.

Following the counting process, a histogram will be automatically generated by counting the average diameter of the cells in the sample.

Analyzing Cell Fluorescence to Determine Transfection Efficiency (GFP, RFP):

It is necessary to assess the transfection effectiveness while introducing the target gene into cells. The reporter gene in a transfected plasmid typically contains the sequence that codes for the fluorescent protein, thus a successfully transfected cell should express the fluorescent protein. Furthermore, one popular technique for analyzing transfection efficacy in lab settings is the use of a flow cytometer or a fluorescence microscope. However, both have drawbacks, including sophisticated operation and expensive cost. With the help of the C100's fluorescence analysis function and the appropriate fluorescence module, it is possible to analyze and automatically count the expression of GFP, RFP, and other fluorescent proteins, making it quick, easy, and efficient to determine the positive rate of target gene expression.

Cell Viability Identification via Cell Fluorescence Analysis (AO/PI Dual Fluorescence Method).

AO/PI dual fluorescence staining, which is frequently used for primary cell counting, has gained popularity as an alternative to trypan blue staining for live cell counting due to its increased accuracy.

Benefits of RWD C100-Pro

FDA Compliance with 21 CFR Part 11

Using account login and permissions settings to ensure data security.

Utilizing electronic signatures and electronic recording to guarantee data integrity.

Smart Cell Recognition

Cells that are clustered can be distinguished and counted individually. The counting result in trypan blue mode will display the percentage of living and dead cells.

The multiple counts standard deviation of the C100 counter is lower.

There is a small divergence between the theoretical value and the actual measured value in a strong linear connection.

The CV value of the C100 counting result is significantly less than 5%, whereas the CV value of hand counting might reach more than 10%.

Quick Cell Counting in about Nine Seconds

As counting 10 samples a day, it can save you more than 24 hours over the course of a month as compared to the hemocytometer.

Manual cell counting with a hemocytometer takes around five minutes; C100-SE/C100 counts cells in under nine seconds. Ten samples counted each day can save over twenty-four hours every month.

Easy-to-Use Functionality

Easy operation is ensured by a touchscreen with integrated user guide interfaces.

Small-Scale Layout

Small size can be used in a variety of settings.

Modular FL cubes that are removable make switching between scenarios simple.