C100/C100-SE Automated Cell Counter

A Real Time-Saver Speeds up Your Research

The RWD automated cell counter C100/C100-SE is a dependable cell counting tool with superior microscope optics and extremely intelligent analysis software. Researchers are relieved of the strenuous daily cell counting job thanks to the device's ability to perform precise, secure cell-counting and fluorescence analysis in less than 9 seconds.

Analyses Are Realized With Various Application Scenarios Using RWD C100 Automatic Cell Counter

Cell Count Analysis: Appropriate for calculating the number of cells or particles with a diameter of 4 to 60 μm, including the majority of cell lines, stem cells, primary cells, etc.

Analysis of Cell Viability: After staining, live cells will be circled in green for cell viability testing in trypan blue mode, and dead cells will be circled in red.

Analysis of Cell Size: Cell size is a crucial component in cell research and is frequently assessed during drug testing, cell transfection, and cell viability.

After counting, it will calculate the sample's average cell diameter and instantly produce a histogram.

Cell Fluorescence Analysis:

Detect Cell Transfection Efficiency (GFP, RFP):

The transfection efficiency must be assessed prior to transfecting cells with the target gene. The reporter gene for the fluorescent protein is often found in the transfected plasmid, therefore a successful transfection should result in the presence of the fluorescent protein in the transfected cell. Moreover, employing a flow cytometer or a fluorescence microscope is a typical way for analyzing transfection efficiency in labs, although both have the drawbacks of complex operation and expensive cost. In order to quickly, easily, and effectively determine the positive rate of target gene expression, the C100's fluorescence analysis function, which is equipped with the necessary fluorescence module, can be used to analyze the expression of GFP, RFP, and other fluorescent proteins and automatically count them.

Cell Fluorescence Analysis:

Identification of Cell Viability (AO/PI Dual Fluorescence Technique).

Trypan blue staining is still extensively used, however AO/PI dual fluorescence labeling has gained popularity as a more precise live cell counting technique.

Features

High Accurate

• The C100 can precisely count and evaluate stem cells, primary cells, and cell lines thanks to a high-definition imaging system and separately created recognition algorithms technology.
• User-defined function. To minimize counting errors, users can establish their own standards for cell size, brightness, and roundness.
• You can change the parameters at any moment to get the best cell imaging and counting results.
• HEK293 and primary mouse macrophages were counted using an automated counter and a hemocytometer. The CV value was less than 5%, and the standard deviation of C100 was less.

Fast

• A count can be finished in 9 seconds in bright field mode, compared to up to 5 minutes for manual counting.
• The operation of fluorescence analysis is simple.
• Built-in dilution calculators can expeditiously provide a dilution solution based on the existing counting result, simplify tests, and save operators a great deal of time.
• Each operator can save 24 hours per month by counting 10 cell samples (5pc) per day.

Convenient

• Numerous data sets, such as cell pictures, diameter distribution histograms, count findings, and the percentage of live and dead cells, are displayed and stored.
• PNG, JPG, CSV, PDF, etc. are useful for analysis and storing.
• The gadget may transfer 1,000 previous counts via USB or store them internally.
• Ergonomic design, minimized visual fatigue and neck pressure, and 45° elevation angle.
• High-definition touch screen that is 7 inches in size, simple to use, and comfy.

Safe

• No need to clean using disposable counting slides, which lowers the risk of biological hazards.