Flaming/Brown Micropipette Puller Sutter P-97

When making micropipettes, patch pipettes, and microinjection needles, the P-97 Flaming/Brown type micropipette puller is the best option. The P-97 includes enhancements in mechanical, electronic, and software design while keeping many of the characteristics of preceding models. Better pulling process control and increased reproducibility are the results. The P-97 blends an advanced, programmable microprocessor controller with a tried-and-true mechanical mechanism. The investigator can create application-specific pipettes from a large variety of glass compositions and sizes thanks to the pulling parameters' adjustable control.

The P-97's design has also included a few more advantages. The environmental chamber that encloses the heating filament is the most noticeable. The purpose of this environmental room is to reduce the impact of fluctuating humidity on the repeatability of drawn pipettes. Using larger heating elements, larger diameter glass, and multi-barreled glass is possible thanks to a 25% boost in power over the P-87. To reduce heat retention, the metal jaws that clasp the heating filament have also been altered. For big diameter and multi-barreled glass, a prolonged cooling period is provided via a gas supply mode switch. For simpler loading of glass, a spring-loaded fastening mechanism has been introduced. There is now a vacuum fluorescent display that makes viewing simple.

A display of the total heat-on time is one of the software upgrades made to the P-97 to help with program management and troubleshooting. The P-97 can now write and store up to 100 programs in memory, making it appropriate for many users. With the addition of write protection, these programs can now be secured against unintentional modification or change. The most recent date and time the script was written or updated are displayed on the screen. Additionally, a customizable setting for air pressure is included.

COMMON APPLICATIONS of P-97

• Patch pipettes
• Sharp electrodes
• Pronuclear injection
• Zebrafish injection
• Insect egg injection
• Aspiration pipettes

FEATURES

• Environmental chamber for controlling humidity.
• Adjustable air pressure.
• Up to 100 programs can be stored in memory.
• Create a protection and date stamp for each script.
• Each draw produces two symmetrical pipettes.
• The two cooling options are time and delay.
• Programs with pre-programmed examples for patch and intracellular pipettes. Special programming is available upon request.
• When a new filament or glass is inserted, the ramp test is a self-test to determine the program heat settings.
• Fluorescent vacuum display.
• Power supply with constant current for the pull solenoid and filament.
• For the manufacture of patch-type micropipettes, use a looping pull cycle.
• Self-contained air supply with humidity control and a filtration system.
• Electrodes with tip sizes less than 0.1 µm that are accurate and predictable.
• Control of the air delivery process, including the timing and pressure.
• A velocity sensing circuit that has been optimized for maximum sensitivity and repeatability.
• SEM image of a tip pulled with each puller for quality control; the requirement is a tip measurement less than 0.1 µm and commonly is 0.06 µm.