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Port-a-Patch

Nanion Port-a-Patch

Port-a-Patch is a turn-key, miniature patch clamp system that lets users quickly produce high-quality data. This makes it the perfect resource for studying patch clamp electrophysiology in an easy-to-use manner.

Appreciate Electrophysiology

Regardless of skill level, the Port-a-Patch is a turn-key, miniature patch clamp system that lets users quickly produce high-quality data. This makes it the perfect resource for studying patch clamp electrophysiology in an easy-to-use manner. Substantial technology, providing high-quality measurements with giga-seals and high success rates in the majority of conventional recording configurations, is concealed behind its modest and compact form. Its versatility and strength can be found in a number of add-ons, giving the user an effective instrument for ion channel research.

Key Features

  • Simple to use, even for non-techies
  • Standard voltage and current clamp
  • Single-channel and whole-cell recordings
  • Adaptable liquid management
  • Temperature regulation (heating and cooling)
  • Suitable with the majority of amplifiers
  • Optional light stimulation
  • Internal production of consumables

Port-a-Patch system operation

It's simple to use the Port-a-Patch; all you have to do is add solutions and cells to the disposable recording chip, and a computer-controlled pump will automatically catch and seal each cell via suction. It is not necessary to have a micromanipulator or microscope.

The range of feasible experiments for scientific research is greatly expanded by Add-Ons like the Internal Perfusion and temperature-controlled External Perfusion, as well as the capacity to do current clamp and bilayer tests.

The Port-a-Patch recording unit, the HEKA amplifier, the computer, and the Suction Control unit make up the Port-a-Patch system. Most of the time, an amplifier that is already available can be integrated with the Port-a-Patch system. Additional accessories like as microscope slides, temperature control, automated perfusion systems, pressure control, and light activation are available for particular tests.

PatchControl Software

The graphical user interface known as PatchControl is used to regulate the parameters required for cell sealing and capture. PatchControl reads critical parameters through creative communication with electrophysiological software, and then proceeds through pre-programmed processes in accordance.

  • Cells can be automatically sealed and captured.
  • Modifications are possible at any time by the user
  • Compatible with the majority of electrophysiology applications, including as WinWCP, pClamp, and PatchMaster

The Port-a-Patch's flexible add-ons increase the range of possible experiments.

  • Temperature-controlled external perfusion
  • Internal perfusion
  • Light stimulation—SOL
  • Suction Control Pro
  • Microscope slide for simultaneous electrophysiology and fluorescence measurements

 

The Port-a-Patch system includes:

  • Port-a-Patch recording station (including Faraday top)
  • Port-a-Patch Suction Control, USB-controlled (no house vacuum needed)
  • EPC-10 USB amplifier (HEKA Electronics), system compatible with other amplifiers
  • Desktop PC or Notebook with pre-installed PatchControl & electrophysiology software
  • NPC-1 borosilicate recording chips
  • Reagent starter kit
  • On-site installation support
  • 1 year warranty with further optional comprehensive service plans available

Specifications of Nanion Port-a-Patch

Average whole-cell stability: ~30 min

Successful whole-cell recordings: 70–90 % (consistent between cell lines)

Throughput: 20–50 data points per day

Chip resistance: 2–3.5 MΩ (customized resistances available)

Seal resistance: >1 GΩ

Series resistance: <10 MΩ

Liquid consumption: ~30 μl/compound

Perfusion time constant: ~150 ms

Minimum exposure time: 250 ms

Internal perfusion time constant: ~5 s

Optical stimulation: Yes (optional)

Current clamp: Integrated as standard

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