CardioExcyte 96 – Electrophysiology, Along with Cell Viability and Contractility

A hybrid device called the CardioExcyte 96 records the electrophysiology and contractility of intact cardiomyocyte networks. Furthermore, as an indicator of both acute and chronic cell activity, the base impedance readout is automatically and continually monitored. Not only do contractile cardiac cells become included, but non-contractile cell types like cancerous or hepatocyte-like cells are also included in the complexities of cytotoxic reactions. High resolution, non-invasive, and label-free measurements of impedance and extracellular field potential are made. Recording from 96 wells at once, the CardioExcyte 96 is an automated instrument.

Key Features:

• Important characteristics:
• Parallel contraction and EFP recordings from the same cells in 96 recording wells
• Physiological and hypoxic environments
• Non-invasive measurements without labels
• Real-time, high-resolution access to beating parameters
• Evaluation of cell activity and toxicity both acutely and over time
• Options for electrical and optical pacing
• Economical consumables

For effective impedance and extracellular field potential measurements, the CardioExcyte 96 is a turn-key system that includes advanced sensor technology and embedded electronics in the well-plate-based consumables. An incubator is not necessary for the CardioExcyte 96 system because it comes with an incubation system. The system is benchtop mounted and has humidity, gas mix, and temperature control.

An excellent software package for data handling and export is a crucial component of the CardioExcyte 96 system, enabling efficiency and user-friendliness. The system has been verified using monolayers or beating 3D-clusters of cardiomyocytes produced from stem cells from all major stem cell providers. It has also been tested using cell lines (cancer cells, hepatocyte-like cells, etc.) for cytotoxicity investigations.

Using a 96-well Format, Observe, Stimulate, Dose, and Analyze Your Cells

Compact hybrid technology, CardioExcyte 96 combines many functional readouts under physiological conditions right on your bench. Automated arrhythmic event recognition enables a quick and precise analysis process across several wells at once. By analyzing the arrhythmic features of hiPSC-CMs, CardioExcyte 96 data enable the prediction of torsadogenic risk due to the system's great sensitivity.

Quick Data Handling and Flexible Data Analysis

With the help of the user-friendly yet highly effective CardioExcyte 96 control software, a wide range of beating parameters may be recorded and analyzed.

• Online tracking of raw data traces, average heart rate, and online analysis on just one screen
• Simple to use and intuitive 
• Variable cardiac readouts: pulse width, rise and fall duration, FPD-max, FPD-zero, amplitude, and beat rate/regularity, among others.
• Readout of base impedance
• Temperature reading from a sensor

DataControl96's automated preloading of recorded files and user-defined analysis templates enables quick and precise analysis as well as consistent procedures.

• Incredibly strong yet user-friendly analytical software
• Quick recalculation of data with a wide range of QC options in response to parameter changes
• Control group normalization or data pooling
• Compound effects that are immediately apparent
• Incredibly flexible database exporting