Advantages of the ELC-01X
- Reduce costs with this more straightforward version of the ELC-03XS
- Conduct juxtacellular fillings and cell staining
- Utilize the accurate current clamp of a bridge amplifier
- Leverage voltage clamp for approaching cells
- Also offered as a module for the EPMS-07 system
- A comparable amplifier without voltage clamp is the BA-01X
- Integrate with npi's miniature headstages for in vivo recording
Amplifier for Extracellular Recording and Electroporation
ELC amplifiers are versatile systems that allow researchers to merge traditional patch and microelectrode recording with electrical stimulation, dye application, or single-cell transfection. All ELC amplifiers enable an investigator to pinpoint a single cell by its distinctive spike train and subsequently electroporate or stimulate this cell in situ. Employing the loosepatch clamp technique for recording and stimulation in slice or in vivo preparations facilitates investigations under more natural, i.e., non-invasive conditions. Experiments can be conducted with minimal harm to individual cells, including subcellular structures such as dendrites and axons. As no gigaseal is necessary for these types of experiments, the same pipette can even be utilized for multiple cells. The ELC features a distinctive headstage with a resistive-feedback circuit capable of operating as follows:
- Patch Clamp headstage with pA sensitivity
- High-impedance electrometer with bridge balance capable of functioning as a conventional bridge amplifier
- Extracellular amplifier with highpass and lowpass filters and gain up to 1000
ELC amplifiers are available in two models:
- The ELC-01X and the ELC-01MX (modular version) feature a straightforward voltage clamp function that facilitates cell approach and seal formation.
- The ELC-03XS serves as a comprehensive patch clamp amplifier designed for whole-cell and perforated patch configurations, boasting enhanced series resistance compensation through an abridge balance circuit.
Consequently, the ELC series amplifiers present an unparalleled blend of functionalities with a single headstage, in contrast to other commercial amplifiers that possess a more restricted functional design. Researchers will discover this amplifier to be an outstanding and highly cost-effective option for executing various recording techniques while employing multiple stimulation protocols.
Features:
- Recording capabilities with patch, sharp, metal, or carbon fiber electrodes
- In vivo recordings utilizing miniature headstages
- Extracellular recordings with elevated gain
- Headstages equipped with additional extracellular channels
- Juxtasomal dye or DNA filling
- Single cell stimulation
- Iontophoresis
- Intracellular recording in CC (bridge) mode using patch or sharp electrodes
- Telegraph lines for filters and gains
- Compatibility with all major data acquisition systems
Specifications of the ELC-01X
Input voltage range: ±12 V
Operating voltage: ±15 V
Enclosure: Size: 23 x 70 x 26 mm, grounded
Dovetail: Size: 70 mm x 17 mm x 3 mm
Electrode connector: BNC with driven shield
REF connector: SMB connector
Ground connector: 2.4 mm connector
Input resistance (CC): >1013 Ω (internally adjustable)
Current range:
- ±100 nA max. (100 MΩ feedback, x1 range)
- ±1.0 µA max. (10 MΩ feedback, x10 range)
- ±10 µA max. (1 MΩ feedback, x100 range)
- ±10 nA max. (1 GΩ feedback, x0.1 range)
OFFSET: range ±100 mV, ten-turn control
CAPACITY COMPENSATION: range 0 – 30 pF, ten-turn control
BIAS: range ±100 pA, ten-turn control
BRIDGE BALANCE: 0-100 MΩ, adjustable with ten-turn control
Sensitivity 1 mV / MΩ application of square current pulses ±1 nA
Display: 3 ½ digit, XXX MΩ, activated by key switch (same as POTENTIAL display)
Full power bandwidth (REL = 0 MΩ): >30 kHz
rise time (10% – 90%)
- <10 µs (REL = 100 MΩ)
- <5 µs (REL = 10 MΩ)
Output impedance: 50 Ω
Max. voltage: ±12 V
Current output: BNC connector, sensitivity 0.1…10 V/nA,
Current output sensitivity: Rotary switch, 0.1, 0.2, 0.5, 1, 2, 5, 10 V/nA
Current display: 3 ½ digits, XX.XX nA, resolution 10 pA
Current LP filter: 4-pole BESSEL filter (other options available)
attenuation: -24 dB/octave
corner frequencies (Hz): 20, 50, 100, 200, 300, 500, 700, 1k, 1,3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k
Potential output x1: BNC connector, sensitivity 1 V/V
Potential output: BNC connector, sensitivity 10…1k V/V
Potential output gain: Rotary switch, 10, 20, 50, 100, 200, 500, 1k
Potential output resolution in AC: 50 µV
Potential LP filter: 4-pole BESSEL filter (other options available)
attenuation: -24 dB/octave
corner frequencies (Hz): 20, 50, 100, 200, 300, 500, 700, 1k, 1,3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k
Potential HP filter: 1-pole filter, (other options available)
attenuation: -6 dB/octave
corner frequencies (Hz): DC, 1, 10, 50, 100, 500
Telegraph potential LP filter -8…+7 V, 1V/step
Telegraph potential HP filter -8…+7 V, 1V/step
Telegraph current filter -8…+7 V, 1V/step
Telegraph potential output sensitivity +1…+7 V, 1V/step
Telegraph current output sensitivity +1…+7 V, 1V/step
Display mV/MΩ 3 ½ digits, XXXX mV or XXX MΩ
Display current 3 ½ digits, XX.XX nA
Input impedance analog 100 kΩ
Input range ±12 V
Input impedance digital (TTL) 10 kΩ
Input range TTL 0-5 V
Current stimulus input CC via BNC connectors, sensitivity 1 nA / V
Current stimulus input CCx10 via BNC connectors, sensitivity 10 nA / V
Step gate input via BNC connector (TTL)
Gated stimulus CC with ten-turn control of holding current resolution: 10 pA, range: ±10 nA
Gated stimulus CCx10 with ten-turn control of holding current resolution: 100 pA, range: ±100 nA
Polarity selectable with toggle switch
Voltage command input VC via BNC connectors, sensitivity: ÷10 mV
Voltage command input VCx10 via BNC connectors, sensitivity: ÷1 mV
Step gate input via BNC connector (TTL)
Gated stimulus VC with ten-turn control of holding potential resolution: 1 mV, range: ±1 V
Gated stimulus VCx10 with ten-turn control of holding potential resolution: 10 mV, range: ±10 V
Polarity selectable with toggle switch
Dimensions: 19” rackmount cabinet, 19” (483 mm), 10” (250 mm), 3.5” (88 mm)
Power requirements: 115/230 V AC, 60/50 Hz, fuse 0.4/0.2 A, slow, 25 W
Weight: 4.0 kg
Ordering Information
- ELC-01X Amplifier for extracellular recording and electroporation
- ELC-MOD Cell Model Circuit for ELC System (passive)
- ELC-SEAL Surplus for Seal Resistance Test
- ELC-PRS Remote Switch for Penetration Unit
- ELC-EH-SET Electrode Holder Set for ELC Amplifiers
- ELC-EH Standard Micro-/Patch Electrode Holder
- ELC-HS ELC Headstage
- ELC-DIFF Surplus for Differential Headstage for ELC Amplifiers
- ELC-SWI Surplus for switchable Headstage, x1/x100
- ELC-MINI Miniature headstage for BA-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options.
Please contact us for further information and prices.
