Holders and Headstages by Axon

At the moment, only Teflon-insulated U-types are available, and they can only be used with HL-U holders and U-type adapters.

It is necessary to order each adapter and holder separately.

HS-2 and HS-2A headstages

These headstages are to be used with GeneClamp and AxoClamp. They are suitable for a variety of applications, including extracellular registration, bath potential measurements, ion-selective measurements, ionophoresis, and intracellular registration on small and large cells. Single voltage amplifiers with variable current amplification make up both headstages. The headstages have circuitry that stops current leakage through the capacitance compensation circuitry for ultra-high impedance electrodes.

Standard headstage options:

• HS-2A-x100MGU
• HS-2A-x1LU
• HS-2A-x10MGU
• HS-2A-x0.1LU
• HS-2A-x1MGU
• HS-2-x0.01MU
• HS-2-x0.0001MU

"M" stands for extended capacitance compensation range.

"G" stands for grounded enclosure.

"A" stands for extended operating voltage range of ±150 V.

HS-4-x1MGU single-amplifier-relay switching headstage

When it is required to maximize electrode voltage with two-electrode potential clamping, this headstage is used in conjunction with Axoclamp. It operates similarly to HS-2 in all other modes. The headstage can only be amplified with a single current (x1MG). The VG-2 headstage is used to measure current.

VG-2 is a virtual earth/ground probe headstage

It can be used in place of or in addition to the Axoclamp to gauge the total bath current. The common gradients are 0.1, 1, and 10. Another gain option is the x100 gain. These headstages' current ranges are 0.1 µA (x0.1), 1 µA (x1), 10 µA (x10), and 100 µA (x100).

VG-2A-x100 bath potential probe headstage

To get a zero potential for the bath, use this along with the Axoclamp or GeneClamp. By doing so, the influence of the bath earthing electrode and the solution's series resistance is eliminated. Reduced solution composition and temperature variations also lessen the impact of gradual potential drift.

Additional CV-5 headstages for GeneClamp 500B

• CV-5-100GU patch-clamp headstagestage (100 mV/pA)
• CV-5B-100GU bilayer patch-clamp headstagestage (100mV/pA)
• CV-5-1GU macro-patch headstagestage (1 mV/pA)
• CV-5-100MU voltammetry headstagestage (100 mV/nA)

These headstages are only compatible with GeneClamp 500B. One feedback resistor, which transforms current to voltage, is present in each headstage. The designations 100G, 1G and 100M are 100GΩ, 1GΩ and 100MΩ resistors. An expanded capacitance compensation range, such as for two layers, is available in the "B" version. For macropatch, 1G is adequate. On carbon microelectrodes, cyclic voltammetry is carried out at 100M.

EX-1 1.5 m extension cable

Connects to HS-2, HS-2A, HS-4, VG-2 and VG-2A headstages.

Mechanical fasteners

The majority of headstages come with an insulated mounting rod that is 102mm long free of charge. You can order 6.3mm or 9.5mm, however 7.9mm is the standard. All headstages also come with an insulated mounting platform that can accommodate a variety of micromanipulators.

Holders

HL-U: For connection to U-headstages. (threaded connection)

HL-U for 1.0-1.7 mm glass

HLB-U BNC adapter

HLR-U corner adapter

The holder can clamp pipettes with an exterior diameter ranging from 1.0 to 1.7mm thanks to four sizes of conical washers. The 1mm tube is joined to the suction tube. The design is low noise and simple to use. Spare parts include five washers of each size, four pieces of 50mm silver wire, and an insulating tube of 70mm silicone. For connecting to the headstage, utilize the BNC connector.

Spare parts for holder HL-UHL-CAP two polycarbonate caps

• HLC-11 orange cone washer, pack of 5 1.1 mm ID, fits 1.0 to 1.1 mm OD glass
• HLC-13 clear cone washer, pack of 5 1.3 mm ID, fits 1.1 - 1.3 mm OD glass
• HLC-15 orange cone washer, pack of 5 1.5 mm ID, fits 1.3 - 1.5 mm OD glass
• HLC-17 clear cone washer, pack of 5 1.7 mm ID, fits 1.5 - 1.7 mm OD glass
• HLP-U 1 mm brass pin, pack of 3 HLT-70 silicone tubing, 1 mm ID, 70 mm long

HL-1: for connection to CV-headstages (not type-U) (unthreaded)

• HL-1-12 for 1.0-1.2 mm glass
• HL-1-17 for 1.5-1.7mm glass
• HLB-1 BNC adapter
• HLR-1 corner adapter

The CV headstages previously used with Axopatch and GeneClamp are compatible with the HL-1 brackets, HLB-1 BNC adapter, and HLR-1 angle adapter. The 1mm tube is connected to the suction tube. C ontains 0.25mm silver wire. Includes spare rubber insulation, spare wire, and five extra rings.

HL-2: for connection to 2mm connectors of non-U type headstages.

• HL-2-12 for 1.0-1.2mm glass
• HL-2-17 for 1.5-1.7 mm glass
• HLB-2 BNC adapter
• HLR-2 angle adapter

To connect to HS-2, HS-2A, HS-4, VG-2, VG-2A, and BH-1 headstages as well as any headstage with a 2 mm plug, use series HL-2, HLB-2 BNC adapter, and HLR-2 angle adaptor. Suction tube and 1mm tube are connected. Contains 0.25mm silver wire. 5 extra rings, extra wire, and rubber insulation are included.

Spare parts for HL-1 and HL-2 holders

• HLO-12 'O' rings fit HL-1-12 and HL-2-12 holders, pack of 10
• HLO-17 'O' rings fit HL-1-17 and HL-2-17 holders, pack of 10
• HLP-O general purpose 2 mm plugs, pack of 5
• HLP-1 1 mm brass pins for HL-1 holders, pack of 3
• HLP-2 2 mm brass pins for HL-2 holders, pack of 3
• HLS-001 pierced rubber seals fit HL-1 and HL-2 holders, pack of 10

For all U and non U type holders

HLA-003 Ag/AgCl tab (pack of 3)

A silver wire-mounted tablet with a 1mm diameter. Teflon tubing insulates the wire from the solvent.

HLA-005 Ag wire (pack of 5)

For use in all holders. 50mm wire diameter 0.25mm.