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Glass Microelectrodes

Capillary Glass & Filaments

Borosilicate capillaries for micropipettes with or without filament are used for making microelectrodes and as sampling devices for biochemical analyses.

In addition to the best micropipette pullers on the market, Sutter Instrument Company offers a large variety of premium capillary glasses in different sizes and materials. Although there are numerous capillary glass sizes and types available, we have carefully chosen just those that meet our standards. You may rely on our knowledge of micropipette technology for accuracy and superior products.

Three different compositions of capillary glass tubing are available from us: quartz, borosilicate, and aluminosilicate. The choice will be based on your application and the skills of your puller. Each composition has its own distinct features.

Fire Polishing

Sutter Instrument sells capillary glass made of borosilicate and aluminosilicate only with fire-polished ends. It is easy to fit into holders thanks to the removal of any sharp edges, and the electrical or mechanical qualities of the glass are unaffected.

Custom Pipettes

Custom pipettes and microtools made by Sutter Instrument are not often offered by other pipette manufacturers. As an illustration, we produce personalized pre-pulled beveled pipettes for microinjection. The custom pipettes are made for research purposes and other than human use, and they are not sterile.

Sizes

With a wide selection of wall thicknesses offered by Sutter Instrument capillary tubing, you can choose the size required for your application. Inside-to-outside diameter ratios typically vary from 0.5 to 0.75mm, with the lower figure denoting thick wall tubing and the upper end denoting thin wall tubing. When all other variables are held constant, capillaries with larger walls yield pipettes with longer tapers and smaller tips, which are better suited for use as intracellular microelectrodes. These pipettes with thicker walls have a tendency to have lower noise contributions from capacitance, making them better suited for patch clamp applications. Larger pore apertures made possible by thinner wall tubing are perfect for microinjection needles and low resistance microelectrodes.

TechNote: What is filamented glass?

The inner wall of a pipette made of filament glass has a small glass rod that has been annealed to it. This rod's capillary action is what allows the pipette to be filled with solution from the back. We advise "filamented-glass" if the resulting pipette tip is less than 1 µl and being used for microinjection or for recording. The filament in the glass helps to prevent air bubbles from forming when solution is added to the pipette, in addition to providing capillary action for rapid filling of the micropipette.

GLASS COMPOSITION

Borosilicate (CORNING 7740)

Glass made from borosilicate is the most popular type. Only TYPE I-CLASS A borosilicate, as defined by ASTM Standard 3.1.2*, is available from Sutter Instrument. When tugged, this glass retains its inside-to-outside ratio for the whole taper length after softening at 821 degrees Celsius. Borosilicate offers a larger operating range and softens at a lower temperature than other glasses. These special qualities enable a wider range of shapes to be employed in chromosome dissection tools, patch pipettes, microinjection needles, and microelectrodes.

Aluminosilicate (SCHOTT 8252)

Compared to borosilicate, aluminum silicate softens at a higher temperature (935 degrees Celsius) and across a much smaller temperature range. As it is drawn, it has a propensity to continuously thin out, allowing for incredibly small tips and short tapers. As an illustration, we have extracted aluminosilicate tips in the 20–30 nm range with 5–6 mm taper lengths. When employed in ion-selective micropipettes, its resistivity is several orders of magnitude higher than borosilicate, minimizing leakage currents. A pipette made of aluminumosilicate, which is tougher than borosilicate, is more suited for piercing dense tissues.

Quartz (HERAEUS HSQ300)

Quartz is the most premium and pure glass that is on the market. With regard to mechanical, electrical, and optical characteristics, it is far superior to all other glasses. It is best for patch clamp recording because it has the lowest dielectric constant, the lowest loss factor, and the maximum volume resistivity. The lowest background noise levels have been attained in single channel patch clamp recordings using quartz due to its chemical purity, which essentially prevents ion leakage (2). The high melting point prevents it from being pulled with standard pullers. However, the Sutter CO2 laser-based P-2000 makes it simple to pull.

* ASTM Designation E438-90 - April 1990

The instrument was used in these investigations:

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