Data Acquisition, Management and Analysis System Dendrite?„?

An amplifier, a computer interface, and data collecting software make up the three tiers of the traditional electrophysiological system architecture. These three layers are combined into practical, completely integrated packages by Sutter Instrument's patch clamp amplifier systems, the IPA® Family, and the dPatch® Amplifier systems, which also contain the increasingly well-liked SutterPatch® Data Acquisition, Management, and Analysis Software. Customers who want to combine an existing amplifier with the features of SutterPatch software can do so with the Dendrite™ solution.

The Dendrite system addresses a wide range of electrophysiological applications with eight analog input signals, four analog output lines, and eight digital outputs at a sampling rate of up to 50kHz. Modern technology, consisting of independent 16-bit A-D and D-A converters, prevents crosstalk and offers sufficient resolution for almost all use case scenarios. Trigger input and output lines allow for equipment coordination.

The High Speed USB 2.0 connection is handy for connecting to the computer, and it takes only a few minutes to install the SutterPatch software and drivers. Most patch clamp and other electrophysiological amplifiers that adhere to the industry standard of +/-10 V signal range can provide input to the Dendrite system. In accordance with accepted standards, it also manages peripherals and amplifiers that accept analog or digital input.

SUTTERPATCH SOFTWARE

The instrument configuration, stimulus application, and external experiment parameters can all be added by the user and time-stamped with the raw data traces using the Dendrite and SutterPatch software system. This comprises the timing and development of the experiment as well as all acquisition parameters. Also, the researcher has the option of manually activating tags to record activities like the application of stimuli in instruments not linked to the Dendrite system.

The raw data can be used to record and store details on sample attributes as well as ambient parameter information. There are support for more than 600 metadata attributes altogether. Animal species, genotype, the day and time a cell sample was prepared, recording solutions, pipette resistance, hardware characteristics, and specifics about the stimuli used are a few examples.

DATA VISUALIZATION AND ANALYSIS

The SutterPatch program was created to make it easier to navigate and analyze large datasets. Several view modes in both a cutting-edge two-dimensional and three-dimensional display are supported by the scope window. The 3D representation is especially helpful for developing assays. SutterPatch, which is built on top of the most recent version of the tried-and-true Igor Pro platform, combines native Igor Pro capability with a plethora of features specifically designed for electrophysiological applications. The patch clamp software will be easy to use for both novice and skilled users with SutterPatch.

Data Acquisition

• Data acquisition is controlled via a fast USB connection
• 8 analog input channels maximum (±10 V; channel sampling rates range from 0.1 to 50 kHz)
• Four (±10 V) analog output channels
• 8 channels of digital output (TTL)
• Overall sampling rate of up to 400kHz
• Sophisticated command waveforms
• An external (TTL) trigger or the onboard microsecond clock can start data gathering

SutterPatch® Software

• Based on the principles of Igor Pro (WaveMetrics, Inc.)
• Routines and paradigms offer total experimental control
• Even the most sophisticated stimulus patterns or custom templates can be easily created with the waveform editor
• All significant data about your experiment is stored in associated metadata
• Procedures for comprehensive data analysis and illustrations fit for publication
• Online line-frequency reduction with quick response
• Runs on Windows 10 or later (64-bit), or Macintosh OS X 10.11 (El Capitan)

Application modules give specific apps specialized functionality.

• A deconvolution technique that excels at identifying small synaptic events even against a noisy backdrop is the Event Detection Module.
• Phase plane plot, timing, and waveform statistics are included in the Action Potential Analysis Module.
• Camera Module: A quick approach to record the characteristics and state of the recorded cell.
• All-points histogram, idealized trace, duration and amplitude distribution, and scatter plot are included in the Single-channel Analysis Module.