BA-03X – Bridge Amplifier

Npi's BA-03X is a intracellular bridge mode and extracellular amplifier, applicable in both perforated and tight seal patch configurations, as well as for electroporation and iontophoresis..

Overview
Specifications
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Benefits of npi's BA-03X

Intracellular bridge mode and extracellular amplifier
The most accurate method for current clamp recording
User-friendly cell penetration mode utilizing sharp microelectrodes
Conduct extracellular recordings or cell staining without the need to change the headstage or pipette
Compatible with any data acquisition system
A more straightforward version of this amplifier is the BA-01X
For enhanced voltage clamp functionality, opt for the ELC-03XS
Pair with our miniature headstages for in vivo recording

The BA amplifiers are high-impedance, wide-bandwidth devices designed for intracellular current clamp recordings using sharp microelectrodes, applicable in both perforated and tight seal patch configurations, as well as for electroporation and iontophoresis. Presently, npi BA amplifiers are utilized in neurons from vertebrates (such as rats, mice, cats, and fish) and invertebrates (including leeches, crabs, snails, and insects), muscle cells, other excitable tissues, and plant cells.

Each amplifier in the BA series is housed within a 19″ rackmount cabinet and features functions such as two ranges for bridge balance, an oscillation shut-off circuit to protect the preparation, and two potential and two current ranges. The amplifier is supplied with a compact headstage that includes a holding bar (or a mounting plate/dovetail adapter upon request) and a BNC connector for the electrode holder, allowing direct attachment to a micromanipulator.

The BA-01X serves as the standard bridge amplifier for intracellular current clamp recordings. With an extended current range (x10, i.e., +/. 120 nA max.), this amplifier is also appropriate for the electroporation of individual cells, facilitating non-invasive, juxtacellular filling of cells with dyes or plasmids.

The BA-03X additionally features high and low pass filters for the recorded potential, along with a potential gain of up to 1000, enabling extracellular recordings as well.

A bridge amplifier is also available as a module for the EPMS-07 system (BA-01M).

Features of the BA-03X

True current clamp functionality that allows for direct measurement of membrane potential while completely canceling out series resistance and stray capacitance
Compatible with sharp microelectrodes or patch pipettes in whole-cell, cell-attached, and perforated-patch configurations
Suitable for extracellular recordings of sub-millivolt signals as well
POTENTIAL OUTPUT GAIN: 10, 20, 50, 100, 200, 500, 1k
POTENTIAL HIGHPASS filter: DC: 0.1, 0.3, 0.5, 1, 3, 10, 30, 50, 100, 200, 500, 600, 1k, 3k Hz
POTENTIAL LOWPASS filter: 20, 50, 100, 200, 300, 500, 700, 1k, 1.3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k Hz
CURRENT OUTPUT SENSITIVITY: 0.1, 0.2, 0.5, 1, 2, 5, 10V / nA
Digital DISPLAYS for current, voltage, and electrode resistance
BUZZ and ELECTRODE CLEAR function, AUDIO monitor
OSCILLATION SHUT-OFF unit to prevent cell damage
TTL gates stimulus input with amplitude adjustment via a 3-digit potentiometer and digital readout of holding current
Enhanced (x10) current range for juxtacellular recordings, electroporation, or iontophoresis

Specifications of the BA-03X Bridge Amplifier

Input voltage range: ±1 V

Operating voltage: ±15 V

Enclosure: Size: 23 x 70 x 26 mm, grounded

Dovetail: Size: 70 mm x 17 mm x 3 mm

Electrode connector: BNC with driven shield

REF connector: SMB connector

Ground connector: 2.4 mm connector

Input resistance (CC): >1013 Ω (internally adjustable)

Current range:

±10 nA max. (100 MΩ feedback, X1 CURRENT RANGE)
±100 nA max. (100 MΩ feedback, X10 CURRENT RANGE)

OFFSET: range ±200 mV, ten-turn control

CAPACITY COMPENSATION: range 0 – 30 pF, ten-turn control

BIAS: range ±150 pA, ten-turn control

BRIDGE BALANCE:

0-100 MΩ (range switch set to 10 MΩ)
0-1000 MΩ (range switch set to 100 MΩ)
adjustable with ten-turn control

Electrode clear circuit: Application of max. DC currents (-Imax or +Imax)

Cell penetration: BUZZ, duration ~2.5 – ~100 ms

Sensitivity 1 mV/MΩ application of square current pulses ±1 nA

Display: 3 ½ digit, XXX MΩ, activated by key switch (same as POTENTIAL display)

Full power bandwidth (REL = 0 MΩ): >30 kHz

rise time (10% – 90%)

<10 µs (REL = 100 MΩ)
<5 µs (REL = 5 MΩ)

Output impedance: 50 Ω

Max. voltage: ±12 V

Current output: BNC connector, sensitivity 0.1…10 V/nA,

Current output sensitivity: Rotary switch, 0.1, 0.2, 0.5, 1, 2, 5, 10 V/nA

Current display:

3 ½ digits, XX.XX nA, resolution 10 pA (CURRENT RANGE X1)
3 ½ digits, XXX.X nA, resolution 100 pA (CURRENT RANGE X10)

POTENTIAL OUTPUT FROM HEADSTAGE: BNC connector, sensitivity 1 V/V

POTENTIAL OUTPUT: BNC connector, sensitivity 10…1k V/V

Potential output gain: Rotary switch, 10, 20, 50, 100, 200, 500, 1k

Potential output resolution in AC: 50 µV

Potential LP filter: 4-pole BESSEL filter (other options available)

attenuation: -24 dB/octave

corner frequencies (Hz): 20, 50, 100, 200, 300, 500, 700, 1k, 1,3k, 2k, 3k, 5k, 8k, 10k, 13k, 20k

Potential HP filter: 1-pole filter, (other options available)

attenuation: -6 dB/octave

corner frequencies (Hz): DC, 0.1, 0.3, 0.5, 1, 3, 5, 10, 30, 50, 100, 300, 500, 800, 1k, 3k

Telegraph potential LP filter -8…+7 V, 1V/step

Telegraph potential HP filter -8…+7 V, 1V/step

Telegraph potential output sensitivity +1…+7 V, 1V/step

Telegraph current output sensitivity +1…+7 V, 1V/step

Display mV/MΩ 3 ½ digits, XXXX mV or XXX MΩ

Display current

3 ½ digits, XX.XX nA (X1 CURRENT RANGE)
3 ½ digits, XXX.X nA (X10 CURRENT RANGE)

Input impedance analog 100 kΩ

Input range ±12 V

Input impedance digital (TTL) 10 kΩ

Input range TTL 0-5 V

Current stimulus input CC

via BNC connectors
X1 CURRENT RANGE: 1 nA/V or 0.1 nA/V
X10 CURRENT RANGE: 10 nA/V or 1 nA/V

Holding current:

X1 CURRENT RANGE: ±10 nA
X10 CURRENT RANGE: ±100 nA
Adjustable with ten-turn control

Step gate input via BNC connector (TTL)

Gated stimulus CC

X1 CURRENT RANGE: resolution: 10 pA, range: ±10 nA
X10 CURRENT RANGE: resolution: 100 pA, range: ±100 nA
with digital control of current step size

Polarity selectable with toggle switch

Dimensions: 19” rackmount cabinet, 19” (483 mm), 10” (250 mm), 3.5” (88 mm)

Power requirements: 115/230 V AC, 60/50 Hz, fuse 0.4/0.2 A, slow, 25 W

Weight: 5.0 kg

Ordering Information

BA-03X BA-03X 19″ Bridge Mode Amplifier
BA-MODA Cell Model /w Active Membrane Resistance for BA System
BA-MOD Cell Model Circuit (passive) for BA System
BA-EH Standard Micro-/Patch Electrode Holder
BA-EH-SET Electrode Holder Set for BRAMP-System
BA-PRS Remote Control f. PENETRATION Unit
BA-PRS-F Remote Control f. PENETRATION Unit (foot)
BA-HS Headstage for BA System
BA-MINI Miniature headstage for BA-series amplifier. Many different configurations available for in-vivo or in-vitro use, housing and cable options.

Please contact us for further information and prices.

You can also visit site of the manufacturer.

BA-03X – Bridge Amplifier

Benefits of npi's BA-03X

Features of the BA-03X

Specifications of the BA-03X Bridge Amplifier

Ordering Information

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