Open-Design Upright Microscope Sutter BOB?„?

The Sutter BOB™ is a straightforward, open-design upright microscope platform perfect for slice electrophysiology, wide field functional imaging, two photon retinal imaging, photostimulation, and new techniques just being developed. It was created to do away with the typical microscope frame. The most basic components of a microscope are an objective and a tube lens. Other parts of the majority of modern microscopes are made to do specific tasks, such as distinct experiment kinds, illumination techniques, and signal detection tools.

By substituting the microscope frame with an optical rail, the possibility to alter the overall height of the microscope is added, which is unheard of in traditional microscope designs. In January, work on slices; in March, perform in vivo tests. The BOB microscope attaches to the "blue rail" with one robust, steady connection and is a small, single assembly. The focus arm and optical rail are joined together for motorized focusing.

An Olympus vertical illuminator, which is incorporated into the base BOB, provides fluorescence epi-illumination. Utilizing the Olympus Oblique Coherent Contrast (OCC) condenser, LED transmitted light illumination is accomplished. The trans-illumination light source is made up of a TLED from Sutter and a TLED controller. A digital signal can trigger the TLED controller, doing away with the requirement for shutters and enabling photostimulation from the trans location. The LED, condenser focus mechanism, and OCC condenser are all simply removed as a single assembly for experiments where transmitted light is not desired. The microscope body can sit considerably lower than a traditional microscope since the transmitted light path is shorter than in other frames. Shorter microscopes are easier to use, more stable, and more ergometrically efficient.

The Sutter BOB fully utilizes Sutter's free Multi-LinkTM software package for micromanipulator positioning when equipped with an optional motorized XY stage or translator with MPC-200 controller. It is typical to search a sizable portion of the slice during whole-cell patch recording in slices to discover relevant neurons. Once you've located your target, if the BOB is set up with Multi-Link, it will then bring your recording and stimulation pipettes into the same field of view so that you may start recording right away. Multi-Link can release the recording pipette and let you relocate the objective and stimulating pipette(s) to the new stimulation zone if you later decide you need to stimulate a region that is outside the present field of vision.

APPLICATIONS

• Patch clamp electrophysiology
• in vivo, in vitro, and slice
• Whole-cell recording
• Intracellular recording
• Material science

FEATURES

• Motorized fixed XY stage or motorized translator are optional
• Microscope with an open design and motorized focus
• Quickly adaptable to experimental requirements
• Optimized for in vivo and in vitro experiments on a single platform
• Specifically made to work with Olympus objective lenses
• The MPC-200's movement is coordinated with micropipette positioning using the free Multi-Link™ software
• Differential Interference Contrast (DIC) or Oblique Coherent Contrast (OCC)
• Epifluorescent lighting